Week

Activity

Objective

 

1

 

Introduction, assignment of mentors, research mentor presentations, research proposal development

 

Development of student-mentor relationships, exposure to research areas

 

2

 

Start of methods workshop-DNA isolation, research presentations, start specific research, discussion of common techniques, resume assignment given

 

 

Scientific speaking practice, work with different biological organisms

 

3

 

Methods-Polymerase Chain Reaction (PCR, restriction digestion of DNA, attend ethics workshop, continue specific research

 

 

Hands-on practice in setting up PCR reactions and thermal cycling

 

 

4

 

Methods-Cloning of DNA fragments and PCR products in plasmid vectors, continue specific research experiments

 

Understand cloning vectors, and methods of cloning

 

5

 

Methods-Southern blotting and hybridization, sequencing, isolate mRNA for Week 6 microarray experiments, attend ethics workshop, continue specific research

 

 

Understand the significance of hybridization to map random DNA fragments to chromosomal locations

 

 

6

 

 

Microarray & Bioinformatics Workshop at DBI, continue specific research

 

 

Understand use of microarrays to screen thousands of genes, understand gene expression

 

 

7

 

 

Bioinformatics Training-Basic DNA sequence analysis, use of molecular databases, BLAST, attend ethics workshop, resume discussion, continue specific research

 

 

Understand sequence analysis, genome sequencing

 

 

8

 

 

Conclude specific research, design poster for display at symposium, career workshop

 

Analyze data and draw conclusions from research

practice in creating scientific presentations

 

9

 

Research Symposium, ethics workshop, conclusion of summer program

 

Poster presentation, discussions, visits by school students and teachers